Dongwook Kim; Rachael R. Jetson; Casey J. Krusemark
Chem. Commun., 2017, 53, 9474-9477
https://doi.org/10.1039/c7cc05236g

Abstract

Here, we describe an immunoassay approach for the detection of enzyme activity by quantitative PCR (qPCR) or parallel DNA sequencing which relies on activity-based probes linked to barcoding DNAs. We demonstrate this technique in the detection of serine hydrolase activities using a fluorophosphonate-oligonucleotide conjugate.