Xiangyan Yi; Lian Xue; Tim Thomas; Jonathan B. Baell
Future Med. Chem., 2020, 12(5), 423-437
https://doi.org/10.4155/fmc-2019-0212

Abstract

Here, we describe our action plan for hit identification (APHID) that guides the process of hit triage, with elimination of less tractable hits and retention of more tractable hits. We exemplify the process with reference to our high-throughput screening (HTS) campaign against the enzyme, KAT6A, that resulted in successful identification of a tractable hit. We hope that APHID could serve as a useful, concise and digestible guide for those involved in HTS and hit triage, especially those that are relatively new to this exciting and continually evolving technology.