Louise Plais; Louis Trachsel; Jörg Scheuermann
Bioconjugate Chem., 2024, XXXX, XXX, XXX-XXX
https://doi.org/10.1021/acs.bioconjchem.3c00559

Abstract

While dual-display DNA-encoded chemical libraries (DELs) are increasingly employed for ligand discovery, some of their fundamental properties have not yet been studied in-depth. Aided with fluorescence polarization experiments, we demonstrate that dual-display DELs are intrinsically asymmetrical entities, and we deduce practical guidelines to perform better-informed on-DNA hit validation from these libraries.