Alexander Roloff; Oliver Seitz
Chem. Sci., 2013, 4, 432-436
https://doi.org/10.1039/C2SC20961F

Abstract

DNA directed chemistry is commonly performed by using nanomolar amounts of DNA templates. Herein we introduce a method that allows the use of attomolar template loads. A DNA templated native chemical ligation yields to the covalent fixation of two fluorophores while the template is being produced during polymerase chain reaction.