Zhengrong Zhu; Alex Shaginian; LaShadric C. Grady; Thomas O'Keeffe; Xiangguo E. Shi; Christopher P. Davie; Graham L Simpson; Jeffrey A. Messer; Ghotas Evindar; Robert N. Bream; Praew P. Thansandote; Naomi R Prentice; Andrew M. Mason; Sandeep Pal
ACS Chem. Biol., 2018, 13, 1, 53-59
https://doi.org/10.1021/acschembio.7b00852

Abstract

A DNA-encoded macrocyclic peptide library was designed and synthesized with 2.4×(10^12) members composed of 4-20 natural and non-natural amino acids. Affinity-based selection was performed against two therapeutic targets, VHL and RSV N protein. Based on selection data some peptides were selected for resynthesis without DNA tag and their activity was confirmed