Fabian Buller; Luca Mannocci; Yixin Zhang; Christoph E. Dumelin; Jörg Scheuermann; Dario Neri
Bioorg. Med. Chem. Lett., 2008, 18, 5926-5931
https://doi.org/10.1016/j.bmcl.2008.07.038

Abstract

DNA-encoded chemical libraries are increasingly being employed for the identification of binding molecules to protein targets of pharmaceutical relevance. Here, we describe the synthesis and characterization of a DNA-encoded chemical library, consisting of 4000 compounds generated by Diels-Alder cycloaddition reactions. The compounds were encoded with unique DNA fragments which were generated through a stepwise assembly process and serve as amplifiable bar codes for the identification and relative quantification of library members.