Yulong An; Hao Yan; Zhenzhen Dong; Alexander L. Satz
Curr. Protoc., 2021, 1(5), e125
https://doi.org/10.1002/cpz1.125

Abstract

An efficient method for the synthesis of DNA‐conjugated 1,2,3‐triazoles is copper (II) [Cu(II)‐β‐cyclodextrin]‐mediated Huisgen cycloaddition (“click reaction”) of DNA‐conjugated alkynes with azides. However, a diverse array of building blocks is required to produce useful DNA encoded libraries, and the commercial availability of azides is limited. The method described herein generates azides in situ from aryl borates and TMSN3, which then further react with DNA‐conjugated terminal alkynes.