Likun Xu; Dongna Zhang; Yuanyuan Dou; Yabin Song; Baogang Wang; Hongquan Wang
J. Int. Pharm. Res., 2018, 45, 10, 736-742
http://202.38.153.236:81/Jweb_jipr/CN/10.13220/j.cnki.jipr.2018.10.002

Abstract

If lead compounds are obtained by high-throughput screening (HTS) of traditional compound libraries, a process that requires the storage and processing of thousands of compounds and the identification of molecules bound specifically to target proteins will be a formidable challenge. DNA Encoding chemical library (DEL) is a group of small organic molecules that bind to DNA tags in a covalent manner and encode molecules with unique DNA markers. This technique allows the construction and screening of combinatorial libraries on an unprecedented scale, through protein target affinity screening and HTS decoding, to efficiently and cheaply discover many unusual affinity ligands. This paper reviews and discusses the characteristics of DEL technology, its construction and screening methods, as well as its application in academic and industrial fields, and the development of DEL and the prospect of the future.