Mary M. Rozenman1; David R. Liu
ChemBioChem, 2006, 7(2), 253-6
https://doi.org/10.1002/cbic.200500413

Abstract

When DNA duplexes are preformed in water, then DNA-templated synthesis (DTS) can take place efficiently and sequence-specifically in organic solvents with low or minimal water content. This simple method enables reactions that are inaccessible in water, such as pyrrolidine-catalyzed aldol condensation, to be performed. The conditions needed to perform DTS in organic solvents also support DNA-templated reactions that are known to take place in aqueous solution.