Dual colour, microarray-based, analysis of 10,000 protease substrates

Juan J. Díaz-Mochón; Laurent Bialy; Mark Bradley
Chem. Commun., 2006, 3984-3986
https://doi.org/10.1039/B609029J

Abstract

A 10,000 member PNA-encoded library of FRET based peptides was synthesised for global analysis of protease cleavage specificity; analysis was achieved using a DNA microarray and consumed minimal quantities of enzyme (60 pmole) and library (3.5 nmole).

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