Kenzo Fujimoto; Kenta Ishida; Li Xue; Shigetaka Nakamura
Photochem. Photobiol. Sci., 2020,19, 776-782
https://doi.org/10.1039/D0PP00098A

Abstract

Ultrafast reversible DNA/RNA photo-cross-linking is a powerful tool for regulating the target strand in living cells. In particular, 3-cyanovinylcarbazole (CNVK) and 3-cyanovinylcarbazole modified by D-threoninol (CNVD) can photo-cross-link to pyrimidine bases within a few seconds of photoirradiation. However, these photo-cross-linkers can only cross-link to the counter base if it is adjacent to the 5′-side (−1 position). In this study, we synthesized novel photo-cross-linkers with varying linker lengths capable of photo-cross-linking with pyrimidine bases at locations other than the −1 position via click chemistry. The photo-cross-linking site was dependent on linker length.