Yaqiong Yan; Lei Wang; Hao Wang
Chem. Res. Chin. Univ., 2023, 39, 83–91
https://doi.org/10.1007/s40242-023-2356-2

Abstract

Combinatorial chemistry provides a cost-effective method for the rapid discovery of new functional peptides. One-bead one-compound(OBOC) high-throughput screening technique offers a lot of structurally diverse peptides to be rapidly synthesized and screened for binding to a target of interest. The OBOC peptide library screening involves three main steps: library construction, positive beads separation, and peptide sequencing. This review mainly summarizes some special technique tips during functional peptide screening and potential future directions of the OBOC high-throughput screening technique.