Nicholas Favalli; Sara Puglioli; Sebastian Oehler; Luca Prati; Jörg Scheuermann; Gabriele Bassi; Samuele Cazzamalli; Dario Neri
Chem. Sci., 2023, 14(43), 12026-12033
https://doi.org/10.1039/D3SC03688J

Abstract

DNA-Encoded Chemical Libraries (DELs) are powerful drug discovery tools, enabling the parallel screening of millions of DNA-barcoded compounds. We investigated how the DEL input affects the hit discovery rate in DEL screenings. Evaluation of selection fingerprints revealed that the use of approximately 105 copies of each library member is required for the confident identification of nanomolar hits, using generally applicable methodologies.