Lynn M. McGregor; David J. Gorin; Christoph E. Dumelin; David R. Liu
J. Am. Chem. Soc., 2010, 132, 44, 15522-15524
https://doi.org/10.1021/ja107677q

Abstract

Interaction-dependent PCR (IDPCR) is a solution-phase method to identify binding partners from combined libraries of small-molecule ligands and targets in a single experiment. Binding between DNA-linked targets and DNA-linked ligands induces formation of an extendable duplex. Extension links codes that identify the ligand and target into one selectively amplifiable DNA molecule. In a model selection, IDPCR resulted in the enrichment of DNA encoding all five known protein−ligand pairs out of 67 599 possible sequences.