Joelle Pelletier; Sachdev Sidhu
Curr. Opin. Biotechnol., 2001, 12, 340-347
https://doi.org/10.1016/S0958-1669(00)00225-1

Abstract

Extremely diverse, DNA-encoded libraries of peptides and proteins have been constructed that include a linkage between each polypeptide and the encoding DNA. Library members can be selected by virtue of a particular binding specificity, and their protein sequence can be deduced from the sequence of the cognate DNA. Such combinatorial biology methods have proven invaluable in both identifying natural protein-protein interactions and also in mapping the specificities and energetics of these interactions in fine detail.