Alexandre Novoa; Takuya Machida; Sofia Barluenga; Anne Imberty; Nicolas Winssinger
ChemBioChem, 2014, 15(14), 2058-65
https://doi.org/10.1002/cbic.201402280

Abstract

Carbohydrate arrays: We report a highly miniaturized synthetic format to access a nucleic‐acid‐encoded hetero‐glycoconjugate libraries with an unprecedented diversity in the combinations of carbohydrates, linkers, and capping groups. Novel information about plant and bacterial lectin specificity was obtained after conversion of this library to a microarray format.
Abstract
Identification of selective and synthetically tractable ligands to glycan‐binding proteins is important in glycoscience. Carbohydrate arrays have had a tremendous impact on profiling glycan‐binding proteins and as analytical tools. We report a highly miniaturized synthetic format to access nucleic‐acid‐encoded hetero‐glycoconjugate libraries with an unprecedented diversity in the combinations of glycans, linkers, and capping groups. Novel information about plant and bacterial lectin specificity was obtained by microarray profiling, and we show that a ligand identified on the array can be converted to a high‐affinity soluble ligand by straightforward chemistry.