Stefan Matysiak; Klaus Hellmuth; Afaf H. El-Sagheer; Arun Shivalingam; Yavuz Ariyurek; Marco de Jong; Martine J. Hollestelle; Ruud Out; Tom Brown
Org. Biomol. Chem., 2018, 16, 48-52
https://doi.org/10.1039/C7OB02119D

Abstract

DNA encoded ligands are self-assembled into bivalent complexes and chemically ligated to link their identities. To demonstrate their potential as a combinatorial screening platform for avidity interactions, the optimal bivalent aptamer design (examplar ligands) for human alpha-thrombin is determined in a single round of selection and the DNA scaffold replaced with minimal impact on the final design.