Peng Zhao; Zitian Chen; Yizhou Li; Dawei Sun; Yuan Gao; Yanyi Huang; Xiaoyu Li
Angew. Chem. Int. Ed. Engl., 2014, 53(38), 10056-9
https://doi.org/10.1002/anie.201404830

Abstract

The selection of DNA-encoded libraries against biological targets has become an important discovery method in chemical biology and drug discovery, but the requirement of modified and immobilized targets remains a significant disadvantage. With a terminal protection strategy and ligand-induced photo-crosslinking, we show that iterated selections of DNA-encoded libraries can be realized with unmodified and non-immobilized protein targets.