Christoph E. Dumelin; Jörg Scheuermann; Samu Melkko; Dario Neri
Bioconjugate Chem., 2006, 17, 2, 366-370
https://doi.org/10.1021/bc050282y

Abstract

DNA-encoded libraries of small organic molecules facilitate the construction of large, encoded self-assembling chemical libraries for the identification of high-affinity binders to protein targets. We have constructed a library of 477 chemical compounds, coupled to 48 mer-oligonucleotides, each containing a unique six-base sequence serving as "bar-code" for the identification of the chemical moiety. The functionality of the library was confirmed by selection and amplification of both high- and low-affinity binding molecules specific to streptavidin.