Jieqiong Qiu; Afaf H. El-Sagheer; Tom Brown
Chem. Commun., 2013, 49, 6959-6961
https://doi.org/10.1039/C3CC42451K

Abstract

Oligonucleotides have been ligated efficiently on solid-phase using CuAAC and SPAAC chemistry to produce up to 186-mer triazole linked DNA products. Multiple sequential ligation reactions can be carried out by using a masked azide approach. This work suggests a novel modular approach to the synthesis of large complex oligonucleotide analogues.