Ying Zhang; Minyu Qi; Dongyao Wang; Yanting Wang; Diya Lyu; Yan Cao
J. Shenyang Pharm. Univ., 2023, 40(4), 529-538
https://doi.org/10.14066/j.cnki.cn21-1349/r.2022.0100

Abstract

Objective: To summarize the strategies of DNA-encoded compound library ( DEL) technology for drug screening．
Methods: Ｒelevant domestic and international literatures were searched, and DEL screening strategies were categorized and elaborated according to the different screening strategy.
Results: The DEL technology mainly included three parts: library construction，compound screening，and hit decoding and reconstitution. The technology of compounds had been a hot research topic in recent years. In order to expand the target scope of DEL screening strategies application，the initial solid-phase of the DEL screening system was developed to liquid-phase，and the target was gradually extended from purified proteins to cell lysates and even live cells． In recent years，various modern technologies were combined with DEL technology，and the affinity-based binding screening were converted to the biochemical activity screening．
Conclusion: DEL technology has a broad prospect，and many research groups and commercial companies are currently conducting further research for DEL screening． It is believed that more breakthroughs in DEL technology and more implementations in drug discovery will be achieved in the future．