Shilian Yang; Yizhou Li
SciencePaper, 2021.4.14
http://www.paper.edu.cn/releasepaper/content/202104-110.

Abstract

This article reports a method of constructing 1,2,4,5-tetrazine compounds by reacting oligonucleotides coupled with nitrile building blocks and hydrazine monohydrate with the participation of thiols. This method has the characteristics of mild conditions and compatibility with water. This research has the following significance: (1) It lays the foundation for the study of oligonucleotide-compatible bioorthogonal reactions based on 1,2,4,5-tetrazine compounds; (2) It is the DNA coding molecular library technology ( DNA encoded library, DEL) to construct a 1,2,4,5-tetrazine molecular library to provide a synthetic method, which increases the structural diversity of the molecular library.