Ravindra Kumar; Afaf El-Sagheer; John Tumpane; Per Lincoln; L. Marcus Wilhelmsson; Tom Brown
J. Am. Chem. Soc., 2007, 129, 21, 6859-6864
https://doi.org/10.1021/ja070273v

Abstract

The copper-catalyzed azide−alkyne cycloaddition reaction has been used for the template-mediated chemical ligation of two oligonucleotide strands, one with a 5‘-alkyne and the other with a 3‘-azide, to produce a DNA strand with an unnatural backbone at the ligation point. A template-free click-ligation reaction has been used for the intramolecular circularization of a single stranded oligonucleotide which was used as a template for the synthesis of a covalently closed DNA catenane.